23 February 2024: Clinical Research
Urinary Concentration of Renal Biomarkers in Healthy Term Neonates: Gender Differences in GST-pi Excretion
Monika Kamianowska 1ACDE* , Agnieszka Rybi-Szumińska 2AD , Aleksandra Kamianowska 2CEF , Mateusz Maciejczyk 3C , Anna Zubrzycka 1B , Joanna Nazarko 1B , Anna Wasilewska 2ACDEDOI: 10.12659/MSM.942819
Med Sci Monit 2024; 30:e942819
Table 1 The characteristics of the immunoassays used to measure the concentrations of the biomarkers.
| Assay working range [ng/ml] | Assay sensitivity [ng/ml] | Assay precision | |||
|---|---|---|---|---|---|
| LLOQ | ULOQ | LOD | Intra-Assay%CV | Inter-Assay%CV | |
| Bio-Plex Pro™ RBM Human Kidney Toxicity Panel 1 | |||||
| Calbindin | 4.4 | 1.570 | 0.97 | 4 | 5 |
| Clusterin | 1.3 | 1.250 | 0.57 | 3 | 10 |
| GST-pi | 0.5 | 230 | 0.23 | 5 | 8 |
| KIM-1 | 0.021 | 21 | 0.01 | 4 | 5 |
| MCP-1 | 0.011 | 3.8 | 0.0017 | 3 | 8 |
| Bio-Plex Pro™ RBM Human Kidney Toxicity Panel 2 | |||||
| B2M | 0.043 | 22 | 0.022 | 3 | 9 |
| LLOQ – lower limit of quantitation; ULOQ – upper limit of quantitation; LOD – limit of detection; CV – coefficient of variation; GST-pi – glutathione-S-transferase-pi; KIM-1 – kidney injury molecule-1; MCP-1 – monocyte chemoattractant protein-1; B2M – beta-2-microglobulin. | |||||