Enhancement of Frozen-Thawed Human Sperm Quality with Zinc as a Cryoprotective Additive

Longlong Fu; Jing Ma; Lixia Chen; Ying Guo; Wenjie Li; Xingguo Zhang; Wenhong Lu; Shusong Wang; Ying Liu

doi:10.12659/MSM.942946

03 May 2024 : Clinical Research

Enhancement of Frozen-Thawed Human Sperm Quality with Zinc as a Cryoprotective Additive

Longlong Fu^1ACEG*, Jing Ma^2AFG, Lixia Chen^3CF, Ying Guo^4BCD, Wenjie Li^5BCDE, Xingguo Zhang^3BCD, Wenhong Lu^1CDG, Shusong Wang^2AFG, Ying Liu^5ACD

DOI: 10.12659/MSM.942946

Med Sci Monit 2024; 30:e942946

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Table 1 Sperm motility parameters for each group (N=13).

	PR (%)	NP (%)	VAP	VSL	VCL
Fresh	31.98±8.18	7.82±2.10	65.03±7.02	46.26±4.15	80.18±8.48
Fresh	0.000^*	0.170	0.004^*	0.001^*	0.013^*
ZnSO – 0 μM	18.35±7.10	6.93±1.62	57.53±4.54	40.74±3.43	73.78±5.03
ZnSO – 0 μM	–	–	–	v	–
ZnSO – 12.5 μM	18.24±6.88	7.32±1.37	57.65±5.67	39.51±3.66	72.79±6.66
ZnSO – 12.5 μM	0.883	0.296	0.956	0.567	0.920
ZnSO – 25 μM	19.26±7.33	7.11±1.82	60.55±6.62	41.42±3.88	77.92±7.42
ZnSO – 25 μM	0.304	0.675	0.093	0.414	0.047^*
ZnSO – 50 μM	18.32±6.74	7.12±1.95	58.3±2.79	40.55±3.25	73.28±3.53
ZnSO – 50 μM	0.448	0.762	0.014^*	0.453	0.838
ZnSO – 100 μM	18.45±7.36	7.42±0.82	57.35±5.95	38.68±5.15	72.05±7.20
ZnSO – 100 μM	0.806	0.344	0.925	0.261	0.672
^* PR – progressive motility; NP – non-progressive motility; VAP – average path velocity; VSL – straight-line velocity; VCL – curvilinear velocity.

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Enhancement of Frozen-Thawed Human Sperm Quality with Zinc as a Cryoprotective Additive

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