26 April 2024 : Laboratory Research
The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in Osteoarthritis
Dawei Geng12BCE, Rongcai Lin1BCD, Peiran Wei1ABF, Cheng Tang1CDF, Yan Xu1BCDF, Liming Wang1AF*DOI: 10.12659/MSM.943738
Med Sci Monit 2024; 30:e943738
Table 1 The primer sequences used for RT-qPCR analysis.
| Gene | Forward primer | Reverse primer |
|---|---|---|
| GAPDH (mouse) | 5-TGACCACAGTCCATGCCATC | 5-GACGGACACATTGGGGGTAG |
| IL-1 beta | 5-TGGAGAGTGTGGATCCCAAG-3 | 5-GGTGCTGATGTACCAGTTGG-3 |
| IL-6 | 5-ACTCACCTCTTCAGAACGAATTG- | 5-CCATCTTTGGAAGGTTCAGGTTG- |
| IL-4 | 5-GGTCTCAACCCCCAGCTAGT-3 | 5-GCCGATGATCTCTCTCAAGTGAT- |
| CD86 | 5-TGTTTCCGTGGAGACGCAAG-3 | 5-TTGAGCCTTTGTAAATGGGCA-3 |
| CCR7 | 5-TGTACGAGTCGGTGTGCTTC-3 | 5-GGTAGGTATCCGTCATGGTCTTG- |
| Arg-1 | 5-CTCCAAGCCAAAGTCCTTAGAG-3 | 5-AGGAGCTGTCATTAGGGACATC-3 |