15 November 2024 : Clinical Research
Adipose-Derived Mesenchymal Stem Cells from Arthritis Patients: Differential Modulation of CD4⁺ T Cell Activation and Cytokine Production
Maciej Ołdak1BC, Weronika Kurowska
1DE, Magdalena Plebańczyk1BE, Iwona Janicka1B, Anna Radzikowska 1CE, Urszula Skalska 1AFG, Ewa Kuca-Warnawin 1ABCDEF*
DOI: 10.12659/MSM.945273
Med Sci Monit 2024; 30:e945273
Table 2 Details of the kits used for the detection of soluble factors in co-culture supernatants.
| Analyte | Test name | Manufacturer | Detection limit |
|---|---|---|---|
| TNF | TNF alpha Human Uncoated ELISA Kit | Invitrogen | 4–500 pg/ml |
| IFNγ | IFN gamma Human Uncoated ELISA Kit | Invitrogen | 4–500 pg/ml |
| IL-4 | IL-4 Human Uncoated ELISA Kit | Invitrogen | 2–200 pg/ml |
| IL-17AF | IL-17 AF (heterodimer) Human Uncoated ELISA Kit | Invitrogen | 30–4000 pg/ml |
| IL-17A | IL-17 A (homodimer) Human Uncoated ELISA Kit | Invitrogen | 4–500 pg/ml |
| IL-17F | IL-17 AF (homodimer) Human Uncoated ELISA Kit | Invitrogen | 16–2000 pg/ml |
| IL-21 | IL-21 Human Uncoated ELISA Kit | Invitrogen | 8–1000 pg/ml |
| IL-22 | IL-22 Human Uncoated ELISA Kit | Invitrogen | 8–1000 pg/ml |
| IL-26 | Human IL-26 ELISA Kit | FineTest | 15.625–1000 pg/ml |
| IL-35 | Human IL-35 ELISA Kit | FineTest | 15.625–1000 pg/ml |
| IL-1Ra | Human IL-1ra/IL-1F3 DuoSet ELISA | R&D Systems | 39.1–2500 pg/ml |
| Galectin 3 | Human Galectin-3 DuoSet ELISA | R&D Systems | 62.5–4000 pg/ml |
| LIF | Human LIF DuoSet ELISA | R&D Systems | 15.6–1000 pg/ml |