13 March 2025 : Review article
Developing Mouse Models for Ovarian Tissue Transplantation and Xenotransplantation: A Review
Feng-xia Liu
EFG 1, Zhong Lin EFG 1, Ka-li Huang A 1*
DOI: 10.12659/MSM.946386
Med Sci Monit 2025; 31:e946386
Introduction
Construction of Mouse Models for Ovarian Tissue Transplantation
Mouse Strains
Ovariectomy Prior to Transplantation
Transplantation Site
Evaluation of Mouse Models for Ovarian Tissue Transplantation
Histological Analysis of the Ovarian Graft
Neovascularization Density
Proliferative Activity of Ovarian Granulosa Cells
Hormonal and Estrous Cycle Monitoring After Transplantation
Timing of Measurements
Future Directions
Conclusions
References
Table 1 Overview of mouse model studies on ovarian tissue transplantation.
| Reference & year | Mouse strains | OTT category | Transplantation site | Key contents |
|---|---|---|---|---|
| Youm et al (2015) []15 | B6D2F1mice | Autologous transplantation | Muscle, sub-renal capsule, subcutaneous tissue | Evaluate the recovery rate, follicle density, and integrity of grafts at different transplantation sites |
| Cheng et al (2022) []16 | SCID mice | Xenotransplantation | Sub-renal capsule | Evaluation of the follicle density, apoptosis index, vascular density, ROS, and the expression of hypoxia and oxidative stress-related genes in the graft |
| Cohen et al (2016) []17 | Nude mice | Xenotransplantation | Muscle | Description of the angiogenic response of the graft and explore the role of endothelial cell Akt1 expression |
| Terren et al (2022) []18 | SCID mice | Xenotransplantation | Subcutaneous tissue | Evaluation of the follicle count, neoangiogenesis, fibrosis, and Ki-67 expression in the graft |
| Nascimento et al (2023) []19 | C57BL/6 mice | Autologous transplantation | Subcutaneous tissue | Evaluation of the estrous cycle of mice after transplantation, graft blood perfusion, follicle count, and expression of transcripts |
| Sanamiri et al (2022, 2023) [,]20 | NMRI mice | Autologous transplantation | Muscle | Evaluation of the condition of ischemia-reperfusion injury in the graft |
| Ebrahimi et al (2024) []22 | NMRI mice | Autologous transplantation | Muscle | Evaluation of histological parameters, inflammation relative to gene expression, and oxidative status in the graft |
| Philippart et al (2021) []23 | SCID mice | Xenotransplantation | Inner wall of the peritoneum | Evaluation of the follicular morphology and ultrastructure of the graft |
| Dolmans et al (2019) []24 | SCID mice | Xenotransplantation | Inner wall of the peritoneum | Evaluation of the oxygen concentration, CD34 endothelial marker, and follicle count in the graft |
| Yan et al (2020) []25 | C57BL/6 mice | Autologous transplantation | Ovarian bursa | Evaluation of the developmental status of follicles at different stages within the graft and the outcomes of the offspring after transplantation |
| Ruan et al (2019) []26 | BALB/c mice | Xenotransplantation | Ovarian bursa cavity, subcutaneous thigh, subcutaneous neck | Evaluation follicular growth and survival, and graft recovery |
| Mahmoudi Asl et al (2021) []27 | BALB/c mice | Autologous transplantation | Subcutaneous tissue | Evaluate the follicular morphology and ultrastructure of the graft, as well as the expression of angiogenic factors and endothelial cell markers |
| Shojafar et al (2019) []28 | NMRI mice | Autologous transplantation | Muscle | Evaluate the ischemia-reperfusion injury of the grafts |