24 June 2025 : Clinical Research
Biomarker Discovery in Childhood Asthma: A Pilot Study of Serum Metabolite Analysis for IgE-Dependent Allergy
Natalia Rzetecka
ABCDEF 1,2, Jan Matysiak ABDEG 1, Szymon Plewa BCDE 1, Joanna Matysiak BDE 3, Paulina Sobkowiak BE 4, Irena Wojsyk-Banaszak BE 4, Anna Bręborowicz BE 4, Weronika Pierz E 5, Agnieszka Klupczyńska-Gabryszak ABCDEF 1*
DOI: 10.12659/MSM.948478
Med Sci Monit 2025; 31:e948478
Table 2 Mass spectrometer parameters.
| Abbreviations | LC-MSa positive | FIAb positive | FIA negative | |
|---|---|---|---|---|
| Scan type | – | MRM | MRM | MRM |
| Duration (min) | – | 9 | 2.9 | 2.9 |
| Curtain gas (psi) | CUR | 20.0 | 20.0 | 20.0 |
| Ion spray voltage (V) | IS | 5500.0 | 5500.0 | −4500.0 |
| Temperature (°C) | TEM | 500.0 | 190.0 | 190.0 |
| Ion source gas 1 (psi) | GS1 | 40.0 | 25.0 | 25.0 |
| Ion source gas 2 (psi) | GS2 | 50.0 | 40.0 | 40.0 |
| Collision gas (psi) | CAD | 6 | 6 | 6 |
| a Liquid chromatography-mass spectrometry; b flow injection analysis. | ||||