24 June 2025 : Clinical Research
Biomarker Discovery in Childhood Asthma: A Pilot Study of Serum Metabolite Analysis for IgE-Dependent Allergy
Natalia Rzetecka
ABCDEF 1,2, Jan Matysiak ABDEG 1, Szymon Plewa BCDE 1, Joanna Matysiak BDE 3, Paulina Sobkowiak BE 4, Irena Wojsyk-Banaszak BE 4, Anna Bręborowicz BE 4, Weronika Pierz E 5, Agnieszka Klupczyńska-Gabryszak ABCDEF 1*
DOI: 10.12659/MSM.948478
Med Sci Monit 2025; 31:e948478
Table 6 Metabolites with statistically significant differences between the study and control group.
| Metabolite | Abbreviation | Class of compounds | Statistical test | P value | Level in the study group [μM] | Level in the control group [μM] | Direction of changes | ||
|---|---|---|---|---|---|---|---|---|---|
| Average±SD | Median (IQR) | Average±SD | Median (IQR) | ||||||
| Serotonin synthesisa | – | – | Mann-Whitney test | 0.011 | 0.014±0.006 | 0.012 (0.003) | 0.020±0.011 | 0.016 (0.006) | Study group ↓ |
| Phosphatidylcholine diacyl C 40:4 | PC 40:4 | Glycerophospholipids | Mann-Whitney test | 0.016 | 2.97±0.53 | 2.97 (0.59) | 2.50±0.37 | 2.61 (0.22) | Study group ↑ |
| Phosphatidylcholine diacyl C 36:0 | PC 36: 0 | Glycerophospholipids | test | 0.020 | 1.06±0.42 | 0.97 (0.61) | 1.91±1.14 | 1.76 (1.70) | Study group ↓ |
| Sphingomyelin (d18: 1/26: 1(17Z)) | SM 44:2/ SM C26:1 | Sphingolipids | test | 0.031 | 0.36±0.08 | 0.33 (0.10) | 0.46±0.12 | 0.49 (0.19) | Study group ↓ |
| Phosphatidylcholine diacyl C 38:0 | PC 38:0 | Glycerophospholipids | test | 0.035 | 2.32±0.77 | 2.15 (1.02) | 3.57±1.80 | 1.76 (1.55) | Study group ↓ |
| Phosphatidylcholine diacyl C 28:1 | PC 28:1 | Glycerophospholipids | test | 0.043 | 2.20±0.38 | 2.15 (0.41) | 2.69±0.69 | 2.77 (1.12) | Study group ↓ |
| Sphingomyelin (d18: 0/16: 1(9Z)(OH)) | SM 35:1/SM (OH) C16:1 | Sphingolipids | test | 0.049 | 2.60±0.42 | 2.61 (0.45) | 3.24±0.99 | 3.15 (1.37) | Study group ↓ |
| a Rate of tryptophan (Trp) conversion to serotonin in 2 steps by tryptophan hydroxylase and aromatic L-amino acid decarboxylase activity. | |||||||||