24 November 2025 : Clinical Research
Perforin Expression and Natural Killer-Cell Proportion as Biomarkers in Secondary Hemophagocytic Lymphohistiocytosis
Jujuan Wang EF 1, Xin Li BE 2, Limin Duan BC 1, Guangli Yin CG 1, Xin Gao AF 1, Hongxia Qiu G 1, Ji Xu AD 1*, Tian Tian
AB 1
DOI: 10.12659/MSM.950615
Med Sci Monit 2025; 31:e950615
Table 2 Proportion of cytotoxic lymphocytes and perforin-positive rates in healthy controls, patients with newly diagnosed secondary hemophagocytic lymphohistiocytosis (sHLH), and patients with sHLH achieving complete response (CR-sHLH).
| Flow cytometric parameters | Healthy controls (n=10) | sHLH (n=19) | CR-sHLH (n=6) | P value (sHLH vs Normal) | P value (sHLH vs CR-sHLH) |
|---|---|---|---|---|---|
| CD8 T cells (%) | 1.21 (0.46–16.44) | 5.39 (1.35–21.73) | 1.43 (0.83–2.07) | 0.317 | 0.023* |
| NK cells (%) | 17.62 (10.24–24.66) | 9.54 (5.84–12.79) | 7.88 (5.11–9.40) | 0.006** | 0.478 |
| CD56 T cells (%) | 19.10 (16.60–22.68) | 23.28 (15.12–27.67) | 32.64 (20.73–38.81) | 0.408 | 0.133 |
| Perforin CD8+ T cells (%) | 17.38 (6.88–34.76) | 30.99 (17.09–55.23) | 37.76 (32.89–53.19) | 0.210 | 0.612 |
| Perforin NK cells (%) | 56.20 (47.58–73.34) | 66.79 (45.89–74.92) | 48.97 (27.58–76.64) | 0.536 | 0.501 |
| Perforin CD56 T cells (%) | 8.39 (3.52–25.01) | 31.60 (19.14–66.64) | 20.54 (12.96–31.19) | 0.035* | 0.245 |
| Data are expressed as median (interquartile range, IQR). CR-sHLH – complete response sHLH; NK – natural killer. * ** | |||||