Threonine in Coronary Artery Disease: Insights From Metabolic Syndrome Amino Acid Profiling

24 June 2026 : Clinical Research

Tomasz Urbanowicz

^{ACDE 1*}, Dagmara Pietkiewicz

^{CDEF 2}, Szymon Plewa

^{CD 2}, Beata Krasińska

^{BCF 3}, Jan Matysiak

^{BCDE 2}, Andrzej Tykarski^{BF 3}

DOI: 10.12659/MSM.951571

Med Sci Monit 2026; 32:e951571

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Table 1 Mobile phase gradient conditions for the determination of the full amino acid panel.

Time (min)	Mobile phase A (%)	Mobile phase B (%)	Flow (mL/min)
0.00	100	0	0.8
0.50	100	0	0.8
1.00	89	11	0.8
6.30	89	11	0.8
12.30	83	17	0.8
14.80	68	32	0.8
14.90	0	100	0.8
16.40	0	100	0.8
16.50	0	100	0.3
17.40	0	100	0.3
17.50	0	100	0.8
17.60	0	100	0.8
17.70	100	0	0.8
19.30	100	0	0.8
19.80	100	0	1.8
20.40	100	0	1.8
20.50	100	0	0.8

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