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15 June 2020: Clinical Research

LncRNA LINC00152 Increases the Aggressiveness of Human Retinoblastoma and Enhances Carboplatin and Adriamycin Resistance by Regulating MiR-613/Yes-Associated Protein 1 (YAP1) Axis

Ying Wang CDE* , Danli Xin ABCE , Lei Zhou BCDF

DOI: 10.12659/MSM.920886

Med Sci Monit 2020; 26:e920886

Figure 2 MiR-613 was a target of LINC00152. (A) The supposed binding sites between miR-613 and LINC00152, and the matched mutant sites of LINC00152 MUT are shown. (B, C) Luciferase activity was measured in Y79 and Weri-RB-1 cells co-transfected with LINC00152 WT or LINC00152 MUT luciferase reporter and miR-613 or miR-NC, respectively. (D–G) RIP and RNA-pull down assays were used to verify the interacted relationship between miR-613 and LINC00152. (H–K) RT-qPCR was conducted to detect the expression levels of LINC00152 and miR-613 in Y79 and Weri-RB-1 cells transfected si-LINC00152#1, si-LINC00152#2, si-LINC00152#3, si-NC, LINC00152, or Vector. ** P<0.01. RT-qPCR – real-time quantitative polymerase chain reaction.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750