15 June 2020>: Clinical Research
LncRNA LINC00152 Increases the Aggressiveness of Human Retinoblastoma and Enhances Carboplatin and Adriamycin Resistance by Regulating MiR-613/Yes-Associated Protein 1 (YAP1) AxisYing Wang CDE* , Danli Xin ABCE , Lei Zhou BCDF
Med Sci Monit 2020; 26:e920886
Figure 3 LINC00152 regulated proliferation, invasion, apoptosis and autophagy of retinoblastoma cells by targeting miR-613. (A–H) Y79 and Weri-RB-1 cells were transfected with si-NC, si-LINC00152#1, anti-miR-NC, anti-miR-613, or si-LINC00152#1+anti-miR-613, untransfected cells as internal control. (A, B) The expression level of miR-613 in transfected Y79 and Weri-RB-1 cells was assessed by RT-qPCR assay. (C, D) CCK-8 assay was performed to evaluate retinoblastoma cell viability at the indicated time points after transfection. (E, F) Cell apoptosis rate and invasion ability were detected in Y79 and Weri-RB-1 cells by flow cytometry and Transwell assays, respectively. (G, H) The protein expression levels of p62, LC3-I, LC3-II and Beclin-1 were examined in Y79 and Weri-RB-1 cells with western blot assay. * P<0.05; ** P<0.01. RT-qPCR – real-time quantitative polymerase chain reaction; CCK-8 – Cell Counting Kit-8.