15 June 2020>: Clinical Research
LncRNA LINC00152 Increases the Aggressiveness of Human Retinoblastoma and Enhances Carboplatin and Adriamycin Resistance by Regulating MiR-613/Yes-Associated Protein 1 (YAP1) AxisYing Wang CDE* , Danli Xin ABCE , Lei Zhou BCDF
Med Sci Monit 2020; 26:e920886
Figure 5 MiR-613 regulated YAP1 expression in retinoblastoma cells. (A) The binding sequences between 3′UTR of YAP1 and miR-613 were predicted by bioinformatics software starBase. (B, C) Dual-luciferase reporter assay was used to assess luciferase activity in Y79 and Weri-RB-1 cells. (D–G) The mRNA and protein expression levels of YAP1 in Y79 and Weri-RB-1 cells introduced with miR-613, miR-NC, anti-miR-613, or anti-miR-NC were measured by RT-qPCR and western blot, respectively. (H) RT-qPCR analysis was used to measure the mRNA expression level of YAP1 in retinoblastoma tissues and normal retina tissues. (I) Correlation analysis between miR-613 and YAP1 was conducted. ** P<0.01. RT-qPCR – real-time quantitative polymerase chain reaction.