17 June 2020: Database Analysis
Coexpression Analysis of the EZH2 Gene Using The Cancer Genome Atlas and Oncomine Databases Identifies Coexpressed Genes Involved in Biological Networks in Breast Cancer, Glioblastoma, and Prostate Cancer
Jin Zhu 1AG* , Lu Jin 1CEF* , Aili Zhang 2BCD* , Peng Gao 1DF , Guangcheng Dai 1BF , Ming Xu 1CF , Lijun Xu 1AD* , Dongrong Yang 1AEG*DOI: 10.12659/MSM.922346
Med Sci Monit 2020; 26:e922346
Figure 6 The relationship between EZH2 and the 185 coexpressed genes. (A) The network constructed using the STRING database. (B) The relationship between EZH2 and the 185 coexpressed genes. (C–I) Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the associations. (C) The quantitative real-time polymerase chain reaction (qRT-PCR) findings show that knockdown of EZH2 with si-EZH2 inhibited expression of CCNB2, MKI67, KIF20A, CCNA2, and CDC20. (D) The knockdown of E2F1 inhibited the expression of EZH2, MCM4, RRM2, DHFR, CCNE2, and CDC25A. These coexpressed genes and EZH2 are regulated by the same gene, E2F1. (E) EZH2 and the coexpressed genes (TOP2A, MYBL2, BUB1, CCNA2, and CDC6) were down-regulated when ANNCA was knocked down. (F) EZH2 and the coexpressed genes, ARUKA, MAD2L1, ZNF367, KPNA2, and TPX2 are regulated by FOXM1, and knockdown of FOXM1 deregulated these genes. (G) Knockdown of HOTAIR deregulated CCNA2, CHEK1, HMMR, NUSAP1, FANCI, and NCAPG. (H) Upregulation of miR-221 promoted the expression of EZH2 and CDC20. (I) Upregulation of miR-101 inhibited the expression of EZH2, STMN, PLK1, and BUB1B. (* P<0.05; ** P<0.01; *** P<0.001).