17 June 2020 : Database Analysis
Coexpression Analysis of the EZH2 Gene Using The Cancer Genome Atlas and Oncomine Databases Identifies Coexpressed Genes Involved in Biological Networks in Breast Cancer, Glioblastoma, and Prostate Cancer
Jin Zhu1AG, Lu Jin1CEF*, Aili Zhang2BCD, Peng Gao1DF, Guangcheng Dai1BF, Ming Xu1CF, Lijun Xu1AD, Dongrong Yang1AEGDOI: 10.12659/MSM.922346
Med Sci Monit 2020; 26:e922346
Figure 7 The effects of siEZH2 in the C4-2 human prostate cancer cell line. (A) The results of cell cycle analysis showed that knockdown of EZH2 resulted in G2/M arrest (B) 23.98% for the control vs. 28.12% for shEZH2 (P<0.05). (C) Single-cell agarose gel electrophoresis for the cell transfected with control or siEZH2. (D) The results showed the tail length of cells transfected with shEZH2 was significantly longer than the cell in the control group (9.9 8±1.51 for the control group vs. 16.31±2.6 for the siEZH2 group). (E) Colony formation assay shows that knockdown of EZH2 inhibited cell proliferation when irradiated, and the results are shown in panel F. (G) Based on The Cancer Genome Atlas (TCGA) prostate dataset, the increased expression of EZH2, MCM4, KIAA0101, MKI67, RRM2, and CDC25A were associated with recurrence or progression after radiation. (* P<0.05; ** P<0.01)