18 October 2020>: Animal Study
Silencing of Long Non-Coding RNA X Inactive Specific Transcript (Xist) Contributes to Suppression of Bronchopulmonary Dysplasia Induced by Hyperoxia in Newborn Mice via microRNA-101-3p and the transforming growth factor-beta 1 (TGF-β1)/Smad3 Axis
Wenhao Yuan 1ABCDEF* , Xiaoyan Liu 1ABCDG* , Lingkong Zeng 1BCDFG* , Hanchu Liu 1ABCDEFG , Baohuan Cai 2ABCDEFG , Yanping Huang 1ABDF , Xuwei Tao 1ABCDF , Luxia Mo 1ABDF , Lingxia Zhao 1BCDF , Chunfang Gao 1BCDFDOI: 10.12659/MSM.922424
Med Sci Monit 2020; 26:e922424
Figure 1 In newborn BPD mice, RAC value and SOD activity were lower and while MDA level was higher. (A) Changes in body weights of BPD newborn mice on the 7th day and the 14th day of BPD modeling, n=5. (B) HE staining showed the variance in lung tissue structure on the 7th day and the 14th day of BPD modeling, with the black arrows indicating alveolar fusion and the red ones indicating alveolar septum, ×400, n=5. (C) RAC was lower in BPD mice on the 7th day and the 14th day of BPD modeling, n=5. (D) Decreased SOD activity and increased MDA level were found in BPD mice, n=3. Two-way ANOVA and Tukey’s multiple comparisons test were applied to determine statistical significance. * p<0.05, ** p<0.01. BPD – bronchopulmonary dysplasia; RAC – radial alveolar counts; SOD – superoxide dismutase; MDA – malondialdehyde; HE – hematoxylin-eosin; ANOVA – analysis of variance.