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18 October 2020 : Animal Research  

Silencing of Long Non-Coding RNA X Inactive Specific Transcript (Xist) Contributes to Suppression of Bronchopulmonary Dysplasia Induced by Hyperoxia in Newborn Mice via microRNA-101-3p and the transforming growth factor-beta 1 (TGF-β1)/Smad3 Axis

Wenhao Yuan1ABCDEF, Xiaoyan Liu1ABCDG, Lingkong Zeng1BCDFG*, Hanchu Liu1ABCDEFG, Baohuan Cai2ABCDEFG, Yanping Huang1ABDF, Xuwei Tao1ABCDF, Luxia Mo1ABDF, Lingxia Zhao1BCDF, Chunfang Gao1BCDF

DOI: 10.12659/MSM.922424

Med Sci Monit 2020; 26:e922424

Figure 1 In newborn BPD mice, RAC value and SOD activity were lower and while MDA level was higher. (A) Changes in body weights of BPD newborn mice on the 7th day and the 14th day of BPD modeling, n=5. (B) HE staining showed the variance in lung tissue structure on the 7th day and the 14th day of BPD modeling, with the black arrows indicating alveolar fusion and the red ones indicating alveolar septum, ×400, n=5. (C) RAC was lower in BPD mice on the 7th day and the 14th day of BPD modeling, n=5. (D) Decreased SOD activity and increased MDA level were found in BPD mice, n=3. Two-way ANOVA and Tukey’s multiple comparisons test were applied to determine statistical significance. * p<0.05, ** p<0.01. BPD – bronchopulmonary dysplasia; RAC – radial alveolar counts; SOD – superoxide dismutase; MDA – malondialdehyde; HE – hematoxylin-eosin; ANOVA – analysis of variance.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750