21 July 2020>: Lab/In Vitro Research
Silencing of Long Non-Coding RNA (LncRNA) Non-Coding RNA Activated by DNA Damage (NORAD) Inhibits Proliferation, Invasion, Migration, and Promotes Apoptosis of Glioma Cells via Downregulating the Expression of AKR1B1Lun Luo ABE* , Chuan Chen ACD , Haiyong He DFG , Meiqin Cai BCFG , Cong Ling BCE
Med Sci Monit 2020; 26:e922659
Figure 1 NORAD was highly expressed in glioma cell lines. (A) The expression of NORAD in glioma cell lines (GSC11, M059J, U251, and T98G) and normal human astrocytes A735 cells was detected using RT-qPCR. Three independent experiments were carried out (n=3). *** P<0.001 vs. A735. (B) The expression of NORAD was tested using RT-qPCR after transfection with si-NORAD-1 or si-NORAD-2. Three independent experiments were carried out (n=3). *** P<0.001 vs. si-NC. NORAD – noncoding RNA activated by DNA damage; RT-qPCR – reverse transcription-quantitative polymerase chain reaction; si-NORAD – small interfering RNAs (siRNAs) against NORAD.