Figure 6 AKR1B1 overexpression reversed the effects of NORAD silencing on proliferation, invasion, and migration of glioma cells. (A) The expression of AKR1B1 was evaluated using RT-qPCR after transfection with AKR1B1 pcDNA3.1. Three independent experiments were carried out (n=3). ** P<0.01 versus pcDNA-NC. (B) CCK-8 assay and (C) colony formation assay tested the proliferation of U251 cells. (D) Invasion of U251 cells was evaluated using Transwell assay (magnification 200×). (E) Representative images of cell migration (magnification 100×). (F) Quantification of cell invasion. (G) Relative quantification, as examined using wound healing assay. All experiments were repeated 3 times independently (n=3). *** P<0.001 vs. si-NC; # P<0.05, ## P<0.01, ### P<0.001 vs. si-NORAD-2+pcDNA-NC. NORAD – noncoding RNA activated by DNA damage; RT-qPCR – reverse transcription-quantitative polymerase chain reaction; pcDNA-AKR1B1 – AKR1B1 overexpressed plasmid pcDNA3.1; pcDNA-NC – empty vector plasmid; CCK-8 – Cell Counting Kit-8; si-NC – negative control for NORAD siRNA; si-NORAD – small interfering RNAs (siRNAs) against NORAD.