21 July 2020>: Lab/In Vitro Research
Silencing of Long Non-Coding RNA (LncRNA) Non-Coding RNA Activated by DNA Damage (NORAD) Inhibits Proliferation, Invasion, Migration, and Promotes Apoptosis of Glioma Cells via Downregulating the Expression of AKR1B1Lun Luo ABE* , Chuan Chen ACD , Haiyong He DFG , Meiqin Cai BCFG , Cong Ling BCE
Med Sci Monit 2020; 26:e922659
Figure 7 NORAD silencing decreased the expression of migration-related genes in glioma cells. The (A) proteins and (B) mRNA expression of MMP2, MMP9, and vimentin was detected using western blotting and RT-qPCR, respectively. All experiments were repeated three times independently (n=3). ** P<0.01, *** P<0.001 versus si-NC; # P<0.05, ## P<0.01 vs. si-NORAD-2+pcDNA-NC. NORAD – noncoding RNA activated by DNA damage; mRNA – messenger RNA; RT-qPCR – reverse transcription-quantitative polymerase chain reaction; si-NC – negative control for NORAD siRNA; si-NORAD – si-NORAD, small interfering RNAs (siRNAs) against NORAD; pcDNA-NC – empty vector plasmid.