21 July 2020>: Lab/In Vitro Research
Silencing of Long Non-Coding RNA (LncRNA) Non-Coding RNA Activated by DNA Damage (NORAD) Inhibits Proliferation, Invasion, Migration, and Promotes Apoptosis of Glioma Cells via Downregulating the Expression of AKR1B1Lun Luo ABE* , Chuan Chen ACD , Haiyong He DFG , Meiqin Cai BCFG , Cong Ling BCE
Med Sci Monit 2020; 26:e922659
Figure 8 (A–C) AKR1B1 overexpression attenuated the effects of NORAD silencing on apoptosis of glioma cells. (A) Flow cytometry analysis detected the apoptotic rate of U251 cells. (B) The expression of apoptosis-related proteins was examined using western blotting. Three independent experiments were carried out (n=3). ** P<0.01, *** P<0.001 vs. si-NC; # P<0.05, ### P<0.001 versus si-NORAD-2+pcDNA-NC. NORAD – noncoding RNA activated by DNA damage; si-NC – negative control for NORAD siRNA; si-NORAD – si-NORAD, small interfering RNAs (siRNAs) against NORAD; pcDNA-NC – empty vector plasmid.