25 August 2020>: Lab/In Vitro Research
Dioscin Attenuates Interleukin 1β (IL-1β)-Induced Catabolism and Apoptosis via Modulating the Toll-Like Receptor 4 (TLR4)/Nuclear Factor kappa B (NF-κB) Signaling in Human Nucleus Pulposus Cells
Longhui Wang DEF , Yuntao Gu BE , Hai Zhao CEF , Rong Chen BD , Wensheng Chen BF , Hao Qi BE , Weisong Gao ABG*DOI: 10.12659/MSM.923386
Med Sci Monit 2020; 26:e923386
Figure 3 Effect of dioscin on IL-1β–activated apoptotic activity in human NP cells. (A, B) Apoptotic activity was evaluated using the TUNEL method. (C–F) Western blotting and quantitative analysis of cleaved caspase-3, -9, and Bcl-2 expressions. (G) Fluorescence immunostaining of cleaved caspase-3 (red) and nucleus (blue). The results are presented as the means ±SD. * P<0.05 relative to the control group; # P<0.05 relative to the IL-1β group, n=5.