14 July 2020>: Clinical Research
Long Non-Coding RNA THOR Enhances the Stem Cell-Like Traits of Triple-Negative Breast Cancer Cells Through Activating β-Catenin Signaling
Binbin Wang 1ABCE* , Qiang Ye 2BCD* , Chuantao Zou 1AEG*DOI: 10.12659/MSM.923507
Med Sci Monit 2020; 26:e923507
Figure 2 THOR knockdown reduces the stemness of TNBC cells. (A) RT-qPCR was constructed to detect the knockdown efficiency of THOR-shRNA in TNBC cells. (B, C) The effects of THOR-shRNA on TNBC cell viability. (D–F) The mRNA and protein levels of stemness markers were examined in TNBC cells with THOR knockdown or not. (G) ALDH1 activity was measured in TNBC cells depicted in (D). (H, I) The capacity of sphere-formation was evaluated in TNBC cells described in (D). ** P<0.01. TNBC – triple negative breast cancer; RT-q-PCR – real-time quantitative polymerase chain reaction; shRNA – short hairpin RNA; mRNA – messenger RNA.