Figure 4 MaR1 suppressed the activation of TLR4 and MyD88 expression in acute lung injury stimulated by THS. Western blot assay and qRT-PCR analyses were adopted to evaluate the relative genes expressions in different groups. (A) TLR4 and MyD88 protein expressions in lung tissues were assessed by western blot assay. TLR4 (B) and MyD88 (C) mRNA levels were determined using qRT-PCR. ** P<0.01 versus Sham; #, ## P<0.01 versus THS+saline group. MaR1 – Maresin-1; THS – trauma hemorrhagic shock; qRT-PCR – quantitative real-time polymerase chain reaction; mRNA – messenger RNA.