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30 August 2020: Animal Study

Promoting Role of Long Non-Coding RNA Small Nucleolar RNA Host Gene 15 (SNHG15) in Neuronal Injury Following Ischemic Stroke via the MicroRNA-18a/CXC Chemokine Ligand 13 (CXCL13)/ERK/MEK Axis

Tiezhu Guo 1AB , Yueting Liu 2CE , Xinliang Ren 1DE , Wei Wang 1EF , Hanrui Liu 3F*

DOI: 10.12659/MSM.923610

Med Sci Monit 2020; 26:e923610

Figure 2 Silencing of SNHG15 inhibits OGD-induced N2a cell apoptosis. N2a cells were subjected to OGD treatment and collected for experiments. (A) Expression of SNHG15, miR-18a, and CXCL13 in cells before and after si-SNHG1 treatment was detected using RT-qPCR. (B, C) Viability of N2a cells was detected using MTT and EdU labeling assays. (D, E) Apoptosis of N2a cells was detected using Hoechst 33258 staining and flow cytometry. Data were shown as mean±SD based on 3 independent experiments. * p<0.05. In panels A, C–E, data were analyzed using one-way ANOVA while data in panel B were analyzed using two-way ANOVA, and Tukey’s multiple comparison test was used for post hoc analysis.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750