30 August 2020>: Animal Study
Promoting Role of Long Non-Coding RNA Small Nucleolar RNA Host Gene 15 (SNHG15) in Neuronal Injury Following Ischemic Stroke via the MicroRNA-18a/CXC Chemokine Ligand 13 (CXCL13)/ERK/MEK AxisTiezhu Guo 1AB , Yueting Liu 2CE , Xinliang Ren 1DE , Wei Wang 1EF , Hanrui Liu 3F*
Med Sci Monit 2020; 26:e923610
Figure 4 Overexpression of miR-18a inhibits apoptosis and promotes viability of N2a cells. (A) MiR-18a expression before and after miR-18a mimic transfection was performed using RT-qPCR. (B) Viability of OGD-treated N2a cells was detected using EdU labeling assay. (C, D) Apoptosis of OGD-treated N2a cells was detected using Hoechst 33258 staining (C) and flow cytometry (D). Data are shown as mean±SD based on 3 independent experiments. * p<0.05. Data were analyzed using the unpaired t test.