30 August 2020>: Animal Study
Promoting Role of Long Non-Coding RNA Small Nucleolar RNA Host Gene 15 (SNHG15) in Neuronal Injury Following Ischemic Stroke via the MicroRNA-18a/CXC Chemokine Ligand 13 (CXCL13)/ERK/MEK AxisTiezhu Guo 1AB , Yueting Liu 2CE , Xinliang Ren 1DE , Wei Wang 1EF , Hanrui Liu 3F*
Med Sci Monit 2020; 26:e923610
Figure 5 The ERK/MEK antagonist reverses the roles of SNHG15 siRNA and miR-18a mimic in N2a cells. An ERK/MEK antagonist, U0126, was introduced into N2a cells with silenced SNHG15 or overexpressed miR-18a. (A) Viability of N2a cells was detected using EdU labeling assay. (B, C) Apoptosis of OGD-treated N2a cells was detected using Hoechst 33258 staining (B) and flow cytometry (C), respectively. Data are shown as mean±SD based on 3 independent experiments. * p<0.05. Data were analyzed using one-way ANOVA.