Figure 3 Upregulation of BDNF inhibited the wound from healing. (A) The mRNA expression level of BDNF in asthmatic and normal HAECs was tested through qRT-PCR. (B) Western blot analysis was performed to measure the relative BDNF protein expressions. (C) Wound closure in the asthmatic group and normal group was tested after exogenous BDNF was added. (D) CCND1 protein expression was assayed via western blot. *** P<0.001 versus Control, ^^^ P<0.001 versus HC, n=3. BDNF – brain-derived neurotrophic factor; mRNA – messenger RNA; qRT-PCR – quantitative real-time reverse transcription polymerase chain reaction; HC – healthy controls.