07 July 2020: Lab/In Vitro Research
Chlamydial-Secreted Protease Chlamydia High Temperature Requirement Protein A (cHtrA) Degrades Human Cathelicidin LL-37 and Suppresses Its Anti-Chlamydial Activity
Xiaohua Dong 1AB* , Wanxing Zhang 2CE* , Jianmei Hou 3DF , Miaomiao Ma 2DF , Congzhong Zhu 4DF , Huiping Wang 2AG , Shuping Hou 2AG*DOI: 10.12659/MSM.923909
Med Sci Monit 2020; 26:e923909
Figure 3 Ab purified endogenous cHtrA can cleave LL-37. LL-37 (2.5 μg) was the substrate in all groups. After incubation with 5, 15, and 45 μL of endogenous cHtrA, as well as the control groups, Western blot assay was performed. Note that the blank control, HeLa S100, untreated protein-G beads, and treated or untreated CT795 beads (coated with anti-CT795 antibodies) all revealed no cHtrA and high LL-37 concentrations, while purified and unpurified L2 HeLa S100 revealed strong cHtrA and weak LL-37 levels in a cHtrA-dose-dependent manner.