07 July 2020 : Laboratory Research
Chlamydial-Secreted Protease Chlamydia High Temperature Requirement Protein A (cHtrA) Degrades Human Cathelicidin LL-37 and Suppresses Its Anti-Chlamydial Activity
Xiaohua Dong1AB, Wanxing Zhang2CE, Jianmei Hou3DF, Miaomiao Ma2DF, Congzhong Zhu4DF, Huiping Wang2AG, Shuping Hou2AG*DOI: 10.12659/MSM.923909
Med Sci Monit 2020; 26:e923909
Figure 3 Ab purified endogenous cHtrA can cleave LL-37. LL-37 (2.5 μg) was the substrate in all groups. After incubation with 5, 15, and 45 μL of endogenous cHtrA, as well as the control groups, Western blot assay was performed. Note that the blank control, HeLa S100, untreated protein-G beads, and treated or untreated CT795 beads (coated with anti-CT795 antibodies) all revealed no cHtrA and high LL-37 concentrations, while purified and unpurified L2 HeLa S100 revealed strong cHtrA and weak LL-37 levels in a cHtrA-dose-dependent manner.