10 August 2020>: Lab/In Vitro Research
Silencing of Long Non-Coding RNA (lncRNA) Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) Protects PC-12 Cells from LPS-Induced Injury via Targeting miR-29a
Yunchao Ban 1ABCD , Cui Cui 2AE*DOI: 10.12659/MSM.923914
Med Sci Monit 2020; 26:e923914
Figure 2 The effect of NEAT1 knockdown on PC-12 cells after LPS treatment. (A) The relative expression of LncRNA NEAT1 after Ad-si-NEAT1 infection. (B) PC-12 cell viability after LPS treatment and Ad-si-NEAT1 infection was detected by CCK-8. (C) Expression levels of inflammatory cytokines after LPS treatment were evaluated by ELISA. (D, E) PC-12 cell apoptosis after LPS treatment and Ad-si-NEAT1 infection was detected by flow cytometry and TUNEL staining. * P<0.05 vs. control,** P<0.01 vs. control, # P<0.05 vs. LPS+si-nc.