10 August 2020>: Lab/In Vitro Research
Silencing of Long Non-Coding RNA (lncRNA) Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) Protects PC-12 Cells from LPS-Induced Injury via Targeting miR-29a
Yunchao Ban 1ABCD , Cui Cui 2AE*DOI: 10.12659/MSM.923914
Med Sci Monit 2020; 26:e923914
Figure 4 Upregulation of miR-29a counteracted the changes induced by Ad-si-NEAT1 in PC-12 cells after LPS treatment. (A) The relative expression of miR-29a in different groups was evaluated by qPCR. (B) PC-12 cell viability after LPS treatment or transduction of Ad-si-NEAT1 or si-miR-29a was assessed by CCK-8. (C–E) PC-12 cell apoptosis was evaluated by flow cytometry and TUNEL staining. (F) Expression levels of inflammatory cytokines was detected by ELISA assay. * P<0.05 vs. control, ** P<0.01 vs. control, # P<0.05 vs. LPS+si-nc, & P<0.05 vs. LPS+si-NEAT1+si-nc.