Figure 1 Knockdown of MLKL downregulated ICAM-1 expression in HUVECs. (A–F) HUVECs were transfected with either negative control or siRNA-MLKL for 24 h. (A, D) mRNA levels of MLKL and ICAM-1 were evaluated using qRT-PCR. Column chart showed the fold differences in the MLKL and ICAM-1mRNA after standardization against the GAPDH mRNA. (B, E) The protein levels of MLKL and ICAM-1 were evaluated by Western blotting and representative Western blot images of MLKL and ICAM-1. (C, F) Protein levels of MLKL and ICAM-1 were evaluated using Western blotting. Column charts show the fold differences in the MLKL and ICAM-1 band intensities after standardization against the band intensity of β-actin. Data are given as mean±SD of 3 independent assays, with each experiment repeated in triplicate. ** P<0.01,*** P<0.001 vs. controls.