13 August 2020: Lab/In Vitro Research
Mixed Lineage Kinase Domain-Like Protein Promotes Human Monocyte Cell Adhesion to Human Umbilical Vein Endothelial Cells Via Upregulation of Intercellular Adhesion Molecule-1 Expression
Fen Cai 12ABC* , Jia-Li Wang 13BC* , Yi-Lin Wu 1C , Yan-Wei Hu 14AEG* , Qian Wang 1G*DOI: 10.12659/MSM.924242
Med Sci Monit 2020; 26:e924242
Figure 2 Overexpression of MLKL upregulated ICAM-1 expression in HUVECs. (A–F) HUVECs were transfected with either control vector pcDNA or pcDNA-MLKL for 24 h. (A, D) mRNA levels of MLKL and ICAM-1 were evaluated using qRT-PCR. Column chart showed the fold differences in the MLKL and ICAM-1mRNA after standardization against the GAPDH mRNA. (B, E) The protein levels of MLKL and ICAM-1 were evaluated by Western blot analysis and representative Western blot images of MLKL and ICAM-1. (C, F) Protein levels of MLKL and ICAM-1 were evaluated using Western blotting. Column charts show the fold differences in the MLKL and ICAM-1 band intensities after standardization against the band intensity of β-actin. Three independent assays (3 replicates for each experiment) were performed (mean±SD). * P<0.05, ** P<0.01, *** P<0.001 vs. controls.