13 August 2020: Lab/In Vitro Research
Mixed Lineage Kinase Domain-Like Protein Promotes Human Monocyte Cell Adhesion to Human Umbilical Vein Endothelial Cells Via Upregulation of Intercellular Adhesion Molecule-1 Expression
Fen Cai 12ABC* , Jia-Li Wang 13BC* , Yi-Lin Wu 1C , Yan-Wei Hu 14AEG* , Qian Wang 1G*DOI: 10.12659/MSM.924242
Med Sci Monit 2020; 26:e924242
Figure 3 MLKL was involved in THP-1 cell adhesion to HUVECs. (A–D) HUVECs were transfected with siRNA-NC or siRNA-MLKL for 24 h, and then cultured together with CFSE-labeled THP-1 cells for 4 h. The number of adherent monocytes was evaluated by fluorescence microscopy. (A, C) Representative images for adherence of THP-1 cells to transfected HUVECs. (B, D) Column charts show the fold differences in THP-1 cell adhesion to transfected HUVECs after standardization against the corresponding controls. Experiments were repeated 3 times. Values are the mean of 3 replicates. * P<0.05, ** P<0.01 vs. Control.