Figure 4 HIF-1α was required in DAAM2-induced invasion of HCC. (A) MG132 (10μM) incubation for 6 hr had no influence on proliferation of HuH7. Proliferation was assessed by MTT assay. (B) Invasion of HuH7 was detected after overexpressing DAAM2. 10 μM MG132 or 10 μM HIF-1α inhibitor BAY87-2243 was used to incubate HuH7. ** Represented P<0.01 compared with control group, and ## represented P<0.01 between indicated groups. Analyzed data were from three independent experiments. (C) Representative images in transwell assay of B. (D) Western blot showed that HIF-1α was knocked down while DAAM2 was overexpressed in HuH7. (E) HIF-1α knockdown extensively decreased the DAAM2-induced invasion. The invasion was detected with transwell assay. ## Represented P<0.01 between indicated groups. (F) Representative images in transwell assay of (E).