Figure 2 The expression of BDNF in the brains of mice sacrificed immediately after exposure to 2D-SWE. The relative mRNA expression of BDNF qRT-PCR (A). The protein levels of BDNF were assessed by western blot (B). The densitometric analysis of each band was performed using Image-Pro Plus 6.0, and protein expression levels were normalized to actin. Data are presented as the mean±SD from 3 independent experiments. Differences in measurement data were compared with one-way ANOVA. * P<0.05; ** P<0.01, and NS – no significance (P>0.05). BDNF – brain-derived neurotrophic factor; 2D-SWE – 2-dimensional shear wave elastography; mRNA – messenger RNA; qRT-PCR – quantitative real-time polymerase chain reaction; SD – standard deviation.