Figure 4 The effects VD3 on inflammatory cells colonic infiltration and NF-κB signaling in DSS-colitis mice treated with rifaximin. (A) Each protein (20 μg) was used for detection of phosphorylated p-P65 and p65. (B) ImageJ software was used to evaluate the integrated band intensity. Data are presented as relative values using corresponding p-P65, p65, and Lamin B as internal reference with the mean±SD. n=5 independent experiments from each mouse. * P<0.05, ** P<0.01.