Figure 4 TPX2 affected NSCLC migration, invasion, and cell replasticity. (A) Relative TPX2 expression analysis in 5 NSCLC cell lines; the ratio of densitometry value to the corresponding GAPDH value was used to reveal the relative protein expression. (B) The upregulation of TPX2 promoted the migration of the A549 cells, whereas its downregulation inhibited the migration of the H1650 cells. (C) The upregulation of TPX2 promoted the invasion of the A549 cells, whereas its downregulation inhibited the invasion of the H1650 cells. (D) Morphological observations of different treated A549 cells in pseudopod and cell rounding (mean±SD; n=3 in triplicate; ** P<0.01).