Figure 1 Differences between A549/DDP cells and parental A549 cells. (A) A549/DDP cells show increased resistance to cisplatin compared with A549 cells, as determined by CCK-8 assays. (B) A549/DDP cells exhibit a fibroblastic morphology, while A549 cells have an epithelial-like appearance. (C–E) Real-time PCR, western blot, and immunofluorescence analyses of gene and protein expression of EMT-related markers E-cadherin and N-cadherin. (F) Migration assays revealed significant enhancement of migration ability in A549/DDP cells relative to A549 cells. (G) Protein expression of Reelin was measured by western blotting. (H) A549 cells were treated with cisplatin (1 μg/mL) for the times indicated, and the protein levels of Reelin were determined by western blotting. * P<0.05, ** P<0.01. CCK-8 – Cell Counting Kit-8; PCR – polymerase chain reaction; EMT – epithelial-mesenchymal transition.