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07 August 2020: Lab/In Vitro Research

Reelin Promotes Cisplatin Resistance by Induction of Epithelial-Mesenchymal Transition via p38/GSK3β/Snail Signaling in Non-Small Cell Lung Cancer

Ji-Min Li 1ABCE* , Fang Yang 1C* , Juan Li 2C , Wei-Qi Yuan 1D , Hao Wang 1BG , Yi-Qin Luo 1DG*

DOI: 10.12659/MSM.925298

Med Sci Monit 2020; 26:e925298

Figure 2 Reelin enhances cisplatin resistance in lung cancer cells. (A) A549/DDP cells were transfected with Reelin siRNA and Reelin expression was examined by western blotting. (B) A549/DDP cells transfected with Reelin siRNA were treated with cisplatin at the different concentrations for 48 hours; the viability was measured by CCK-8 assays. (C) A549/DDP cells were transfected with Reelin siRNA, treated with 4 μg/mL cisplatin for 24 hours, and stained with Annexin V-FITC and propidium iodide. Cell apoptosis was analyzed by flow cytometry. (D) A549 cells were transfected with the Reelin expression vector, and Reelin expression was evaluated by western blotting. (E) A549 cells transfected with the Reelin expression vector were treated with cisplatin at the indicated concentrations for 48 h; cell viability was determined by CCK-8 assays. (F) A549 cells were transfected with the Reelin expression vector, treated with 2 μg/mL cisplatin for 24 hours, and stained with Annexin V-FITC and propidium iodide. Cell apoptosis was analyzed by flow cytometry. (G) H1299 and H460 cells were transfected with Reelin, and cell viability was assessed by CCK-8 assay. * P<0.05, ** P<0.01. CCK-8 – Cell Counting Kit-8.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750