Figure 5 Activation of the p38/GSK3β signaling pathway is involved in Reelin-induced EMT and cisplatin resistance in A549 cells. (A) A549 cells were transfected with the Reelin expression vector, and p-AKT, p-p38, p-JNK, p-ERK, β-catenin, and p-GSK-3β were evaluated by western blotting. (B) A549/DDP cells were transfected with Reelin siRNA, and p-p38 and p-GSK-3β were evaluated by western blotting. (C) A549 cells transfected with the Reelin expression vector were treated with DMSO or p38 inhibitor SB203580 (10 μM) for 48 hours, and p-GSK3β, Snail, and EMT markers were evaluated by western blotting. (D) A549 cells were treated with LiCl (40 mM) for 6 hours. The expression of Snail and p-GSK-3β were analyzed by western blotting. (E) Cell migration was measured by Transwell migration assays. (F) Cell viability was analyzed by CCK-8 assays. * P<0.05, ** P<0.01. CCK-8 – Cell Counting Kit-8.