02 September 2020: Clinical Research
miR-22-3p Suppresses Endothelial Progenitor Cell Proliferation and Migration via Inhibiting Onecut 1 (OC1)/Vascular Endothelial Growth Factor A (VEGFA) Signaling Pathway and Its Clinical Significance in Venous Thrombosis
Huoqi Liang ABDE* , Yibiao Chen BCDF* , Hefei Li CDF , Xiaoqiang Yu CEF , Chunqiu Xia CF , Zhibing Ming AEG* , Chongjun Zhong AFG*DOI: 10.12659/MSM.925482
Med Sci Monit 2020; 26:e925482
Figure 4 MiR-22-3p negatively regulates the expression of vascular endothelial growth factor A (VEGFA). (A) VEGFA messenger ribonucleic acid (mRNA) expression detected by quantitative real-time polymerase chain reaction (qRT-PCR) in different groups. (B) VEGFA protein expression analyzed by Western blot in different groups. (C) Quantitative analysis of VEGFA protein level in endothelial progenitor cells (EPCs) transfected with different duplexes. (D) Cell-Counting Kit 8 (CCK-8) assay showed the proliferative ability of EPCs in different groups. (E) Transwell assay revealed migration of EPCs transfected with either small interfering (si)RNA control or OC1 siRNA. (F) Wound-healing assay detected the migration ability of EPCs in different groups. * P<0.05; ** P<0.01.