06 September 2020 : Animal Research
Biological Network Model of Effect of Chronic Intermittent Hypoxia on Spermatogenesis in Rats
Jisheng Wang12ABDE, Xuefeng Gong2ABEF, Fanchao Meng12ABCDEF, Sheng Deng12CDF, Hengheng Dai12BF, Binghao Bao12BE, Junlong Feng12BF, Haisong Li2AG*, Bin Wang2AEDOI: 10.12659/MSM.925579
Med Sci Monit 2020; 26:e925579
Figure 1 Study flowchart. Based on DAVID, we predicted the proteins and pathways associated with OSAHS and oligozoospermia. We used qPCR and Western blotting to measure expression of leptin, JAK, and STAT proteins and mRNA in the predicted pathways in rat testis cells from each group. The results of in vivo experiments were consistent with the results predicted by bioinformatic analysis. Chronic intermittent hypoxia and oligozoospermia were highly correlated with proteins in the JAK pathway. Hypoxia was able to activate the JAK2/STAT6 signaling pathway, inhibit cell proliferation, and promote apoptosis, which led to pathologic damage to rat testes and caused a reduction in the count and motility of sperm.