Figure 8 Analyses of testicular tissue in rats using H&E staining. The testicular tissue of rats was stained with hematoxylin & eosin (H&E) to observe pathologic changes in the testes under electron microscopy (n=6 animals per group). In Group A: (i) The convoluted tubules are closely arranged, and the boundary between the basal membrane and the stroma is clear and even (Arrow ➀); (ii) All levels of spermatogenic cells and spermatogenic cells are arranged in order (Arrow ➂➃➆). In Group B: (i) Spermatogenic tubules are damaged to different degrees, and the number and morphology of spermatogenic tubules are significantly changed (Arrow ➄➇); (ii) The convoluted tubules are sparsely arranged, interstitial edema is observed, and the epithelium of the tubules is separated from the stroma; the convoluted tubules have a cavity-like structure (Arrow ➁); (iii) The degeneration, nuclear shrinkage, and necrosis of spermatogenic cells and spermatogenic cells in the convoluted tubules is seen (Arrow ➅➈); (iv) All levels of spermatogenic cells have disappeared and proliferation of interstitial cells is seen (Arrow ➈).