23 June 2020 : Laboratory Research
A PD-L1 Aptamer Selected by Loss-Gain Cell-SELEX Conjugated with Paclitaxel for Treating Triple-Negative Breast Cancer
Xiaoqiu Wu123BEF, Fangfei Li123AG, Yongshu Li1BF, Yuanyuan Yu13DF, Chao Liang13C, Baoting Zhang4DE, Chuanzong Zhao56B, Aiping Lu12378AG, Ge Zhang123AG*DOI: 10.12659/MSM.925583
Med Sci Monit 2020; 26:e925583
Figure 4 Identify the specific aptamer targeting PD-L1 over-expressed MDA-MB-231 cells. (A) Flow cytometric assay showing the binding of XQ-P3 or RS to MDA-MB-231 PD-L1 OE and MDA-MB-231 PD-L1 KO cells. The DNA concentration is 250 nM. (B) Confocal microscopy images of XQ-P3 (250 nM) in 2 MDA-MB-231 cell lines. Scale bar=100 μm. (C) Secondary structure of XQ-P3 predicted by NUPACK. (D) The dissociation constant of XQ-P3 binding with MDA-MB-231 PD-L1 OE cells was determined by flow cytometry (n=3). RS, a random sequence served as a negative control of XQ-P3 aptamer.