23 June 2020>: Lab/In Vitro Research
A PD-L1 Aptamer Selected by Loss-Gain Cell-SELEX Conjugated with Paclitaxel for Treating Triple-Negative Breast Cancer
Xiaoqiu Wu 123BEF* , Fangfei Li 123AG** , Yongshu Li 1BF , Yuanyuan Yu 13DF , Chao Liang 13C , Baoting Zhang 4DE , Chuanzong Zhao 56B , Aiping Lu 12378AG* , Ge Zhang 123AG*DOI: 10.12659/MSM.925583
Med Sci Monit 2020; 26:e925583
Figure 5 PD-L1 is the target protein for XQ-P3. (A) Internalized assay of aptamer XQ-P3 in MDA-MB-231 PD-L1 OE cells after incubation at 37°C for 2 hours. (B) Different concentrations of XQ-P3 based PD-L1 pull down assay. His tagged PD-L1 protein was immobilized on the Ni-NTA magnetic agarose beads and then incubated with the indicated concentration of DNA.