Figure 3 (A) Scatter plot of PLS-DA scores of the first principal component obtained from (■) controls and (●) ASD. t[1]p – PLS component 1; t [2]p – PLS component 2. (B) Validation of the corresponding partial least-squares discriminant analysis model by random permutation analysis. (C) The corresponding loading plots represent single NMR spectral region segments. The color map shows the significance of metabolite variations between the 2 clusters.