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15 December 2020: Clinical Research

Measurement of Cyclin D2 () Gene Promoter Methylation in Plasma and Peripheral Blood Mononuclear Cells and Alpha-Fetoprotein Levels in Patients with Hepatitis B Virus-Associated Hepatocellular Carcinoma

Yu Qian 1ABCDEF , Jing-Wen Wang 1BC , Yu-Fang 1BCD , Xiao-Dong Yuan 1B , Yu-Chen Fan 12ADF , Shuai Gao 12CDFG , Kai Wang 12ADEG*

DOI: 10.12659/MSM.927444

Med Sci Monit 2020; 26:e927444

Figure 3 Methylation status of CCND2 promoter and correlation with expression of CCND2 mRNA. (A) In total, 55/118 (46.61%) hepatocellular carcinoma (HCC) patients, 8/47 (17.02%) liver cirrhosis (LC) patients, 8/75 (10.67%) chronic hepatitis B (CHB) patients, and 4/35 (11.43%) healthy controls (HCs) exhibited aberrant CCND2 promoter methylation in PBMCs. (B) 52/118 (44.07%) HCC patients, 5/47 (10.64%) LC patients, 7/75 (9.33%) CHB patients, and 2/35 (5.71%) HCs exhibited aberrant CCND2 promoter methylation in plasma. (C) CCND2 mRNA levels differed significantly between the methylation group and non-methylation groups (P<0.05). (D) Representative measurements of CCND2 promoter by methylation-specific polymerase chain reaction (MSP). PC – positive control; WB – water blank; M – methylated sequence; U – unmethylated sequence.

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Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750