Figure 3 Effect of SIN on expressions of IFN-γ and IL-4 in decidual and placental tissues of RSA mice. (A) The mRNA levels of IFN-γ and IL-4 in the control, RSA, and SIN-treated mice were examined using RT-qPCR, and the IFN-γ/IL-4 ratio was calculated. (B) The protein expression levels of IFN-γ and IL-4 were detected by western blot analysis. Experiments were performed in 3 independent replicates with essentially identical results, and representative results are shown. (C) Densitometer analysis results for IFN-γ, IL-4, and IFN-γ/IL4 ratio. Data are presented as mean±SEM. * P<0.05, compared with the control group; # P<0.05, compared with the RSA group.