Figure 5 Effect of SIN on expressions of T-bet and GATA-3 in decidual and placental tissues of RSA mice. (A) The mRNA levels of T-bet and GATA-3 were examined using RT-qPCR and the T-bet/GATA-3 ratio was calculated. (B) The protein expression levels of T-bet and GATA-3 were detected by western blot analysis. Experiments were performed in 3 independent replicates with essentially identical results, and representative results are shown. (C) Densitometer analysis results for T-bet, GATA-3, and T-bet/GATA-3 ratio. Data are presented as mean±SEM. * P<0.05, compared with Control group; # P<0.05, compared with RSA group.