Figure 5 Analyses of testicular tissue in mice using hematoxylin-eosin (H&E) staining. The testicular tissue of mice was stained with H&E to observe pathologic changes in the testes under electron microscopy (n=6 animals per group). In group A: (i) The convoluted tubules are closely arranged, and the boundary between the basal membrane and the stroma is clear and even (arrow ?); (ii) all levels of spermatogenic cells and spermatogenic cells are arranged in order (arrows ?, ?). In group B: (i) Spermatogenic tubules are injured to different degrees, and the seminiferous tubules were sparsely arranged, showing interstitial edema, and their epithelium was separated from the matrix (arrow ?); (ii) the number of Sertoli cells in testes was significantly reduced and arranged loosely. The number of spermatozoa was significantly reduced (arrows ?, ?).