01 May 2021 : Laboratory Research
Thrombin Aggravates Hypoxia/Reoxygenation Injury of Cardiomyocytes by Activating an Autophagy Pathway-Mediated by SIRT1
Xiaoning Wang1A, Yunhe Xu2BC, Lingbo Li3F, Weiwei Lu1EF*DOI: 10.12659/MSM.928480
Med Sci Monit 2021; 27:e928480
Figure 1 Induction of inflammatory factor secretion and oxidative stress by thrombin in H/R-injured cardiomyocytes. (A) MDA content, ROS level, and SOD activity were detected to evaluate oxidative stress in the H/R model treated with thrombin, the H/R model treated with dabigatran, and the H/R model treated with a combination of thrombin and dabigatran, as well as the controls. (B) ELISA was used to determine the level of IL-1b, IL-6, TNF-a, ICAM-1, and MMP-9. (C) MTT was conducted to test cell viability. *** P<0.001 vs control group, n=3.